Reagents of Gram Staining

So in the previous post we learned about the Principle of Gram Staining  as i promised i’ll discuss about other things related to gram staining so here it is.Today we are going to discuss the Reagents of Gram staining and their role in Gram staining .

Four reagents are used in this staining.

  • Crystal violetGram + and Gram - difference
  • Gram iodine
  • Acetone iodine
  • Diluted carbol fuchsin/safaranin

Now discussing them one by one.

Crystal Violet : 

It’s the stain which gives purple color to the slide.

Gram iodine :

It is a mordant.A mordant is a substance used to set dyes on fabrics or tissue sections by forming a coordination complex with the dye which then attaches to the fabric or tissue.

Acetone Iodine:

Acetone iodine is the decolorizer which removes the stains/dyes on the slide.

Diluted carbol fuchsin/safaranin:

They are the counter stains i-e the stain the background material that can be dust particles or other organisms.So that we can differentiate between the organism we want to see and the background substances.


So here it is.That’s another post related to gram staining.We’ll discuss about the Procedure of Gram Staining and interpretation of the results in next posts.

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